Extranodal NK/T Cell Lymphoma, Nasal Type
Supplemental Studies
Immunohistology and Flow
NK type
| CD56 |
100% |
| EBV LMP |
Positive, less sensitive than in situ hybridization |
| CD3 |
Cytoplasmic positive, membrane negative |
| Cytotoxic granule proteins: granzyme B, TIA1, perforin |
Generally positive |
| CD43, CD45RO |
Generally positive |
| CD20, CD79a |
Rare positive cases reported |
| CD30 |
Occasionally positive |
| CD4, CD5, CD8 |
Negative |
T cell type
| CD56 |
Negative |
| CD3 |
Positive |
| CD5, CD8 |
May be positive |
| Cytotoxic granule proteins: granzyme B, TIA1, perforin |
Required for diagnosis |
| EBV |
Required for diagnosis |
- CD56 is not specific, it may be seen in other lymphomas and non-lymphoid neoplasms
Genetic analysis
- EBV EBER positive by in situ hybridization 25-95%
- Depends on geographic location
- In endemic areas 95%
- Cases with NK phenotype will have germline T cell receptor gene configuration
